RGDSP功能化肽水凝膠，促進人羊膜間充質干細胞創面愈合

2024年10月，貴州省細胞工程重點實驗室，遵義醫學院附屬醫院,遵義醫科大學附屬醫院臨床干細胞研究所，湖北醫科大學附屬太和醫院創面修復與皮膚外科 (Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi, China,The Clinical Stem Cell Research Institute, Affiliated Hospital of Zunyi Medical University, Zunyi, China,Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, China) Wei Wei老師研究團隊在《Frontiers in Bioengineering and Biotechnology》上發表論文：

“RGDSP-functionalized peptide hydrogel stimulates growth factor secretion via integrin αv/PI3K/AKT axis for improved wound healing by human amniotic mesenchymal stem cells"

“RGDSP功能化肽水凝膠通過整合素αv/PI3K/AKT軸刺激生長因子分泌，促進人羊膜間充質干細胞創面愈合"

Abstract：

The wound healing process involves communication among growth factors, cytokines, signaling pathways, and cells in the extracellular matrix, with growth factors acting as key regulators. Although stem cells can promote wound healing by secreting diverse growth factors, their therapeutic potential is hindered by poor survival and engraftment. Mimicking the stem cell-matrix interactions can improve stem cell survival, regulate their fate, and even enhance their paracrine effects. This study investigated the use of composite RGDmix hydrogel, which can support the survival and proliferation of human amniotic mesenchymal stem cells (hAMSCs), and effectively increase the expression of various growth factors, thereby promoting wound re-epithelialization, angiogenesis, and epidermal maturation. At last, the specific role of integrin αv and PI3K/AKT signaling pathways in the secretion of growth factors were examined by silencing them in vitro and in vivo. Results suggested that the RGDmix hydrogel improved the secretion of growth factors by hAMSCs through the RGDSP/integrin αv/PI3K/AKT axis, thereby enhancing the therapeutic effect in wound healing.

摘要：

傷口愈合過程涉及生長因子、細胞因子、信號通路和細胞外基質細胞之間的交流，其中生長因子是關鍵的調節因子。雖然干細胞可以通過分泌多種生長因子促進傷口愈合，但其治療潛力受到生存和植入性差的阻礙。模擬干細胞與基質的相互作用可以提高干細胞的存活率，調節其命運，甚至增強其旁分泌作用。本研究研究了復合RGDmix水凝膠能夠支持人羊膜間充質干細胞（hAMSCs）的存活和增殖，并有效增加各種生長因子的表達，從而促進創面再上皮化、血管生成和表皮成熟。最后，通過體外和體內實驗，研究整合素αv和PI3K/AKT信號通路在生長因子分泌中的具體作用。結果表明，RGDmix水凝膠通過RGDSP/integrin αv/PI3K/AKT軸促進hAMSCs分泌生長因子，從而增強傷口愈合的治療效果。

該論文中，hAMSCs的體外培養是使用Ausbian特級胎牛血清完成的。