DDIT3對Luminal A型乳腺癌的影響

2023年7月，黑龍江省科學院先進技術研究所;黑龍江省科學院先進技術研究所(Institute of Advanced Technology, Heilongjiang Academy of Sciences；Institute of Advanced Technology, Heilongjiang Academy of Sciences) Guoqing Huang老師研究團隊在《Research Square》上發表論文：

“The effect of DDIT3 on luminal A type breast cancer"

“DDIT3對Luminal A型乳腺癌的影響"

Abstract：

Purpose: To analyze the phenotypic changes of breast cancer (BC) cell before and after DDIT3 knockdown/overexpression, and preliminarily explore the regulatory mechanism. Also, to analyze the relationship between DDIT3 and prognosis by combining with bioinformatics methods, which provide a basis for further research on DDIT3 targeted treatment of BC.

Methods: Loss- and gain-of-function studies, DDIT3 in MCF-7 (luminal A), and RNA-seq analysis were employed to investigate the functional impact of DDIT3 on BC cell proliferation and drug resistance. The relationship between DDIT3 and the prognosis of BC patients was systematically assessed using the tissue microarray technique along with qRT-PCR and publicly available data.

Results: Survival analysis showed that patients with lower DDIT3 expression in luminal A type BC or BC patient which were undergoing endocrine therapy had a poorer prognosis, and DDIT3 expression was associated with overall survival (OS) significant. Following the knockdown of DDIT3 in MCF-7 cells, the proliferation rate was significantly increased, and drug resistance ability was just reversed. On the contrary, overexpression of DDIT3 had a relative inhibitory effect on target cell proliferation. Notably, the inhibition of DDIT3 expression upregulated TP63 and downregulated PDGFR, with the results being exactly opposite after the overexpression of DDIT3.

Conclusion: These results have revealed that DDIT3 plays a critical role in luminal A type BC cell proliferation and TAM resistance, and it holds potential prognostic value in BC. Overall, DDIT3 may exert its functions in luminal A type BC by modulating the expression of TP63 and PDGFR.

摘要：

目的:分析乳腺癌(BC)細胞DDIT3敲低/過表達前后的表型變化，并初步探討其調控機制。結合生物信息學方法分析DDIT3與預后的關系，為進一步研究DDIT3靶向治療BC提供依據。

方法:通過功能缺失和功能獲得研究、MCF-7 (luminal A)中的DDIT3和RNA-seq分析來研究DDIT3對BC細胞增殖和耐藥的功能影響。利用組織微陣列技術、qRT-PCR和公開數據系統評估DDIT3與BC患者預后的關系。

結果:生存分析顯示，在Luminal A BC或接受內分泌治療的BC患者中，DDIT3表達較低的患者預后較差，且DDIT3表達與總生存(OS)顯著相關。MCF-7細胞中敲低DDIT3后，增殖速率明顯提高，耐藥能力剛好逆轉。相反，過表達DDIT3對靶細胞增殖有相對抑制作用。值得注意的是，抑制DDIT3表達可上調TP63，下調PDGFR，而過表達DDIT3后的結果正好相反。

結論:這些結果揭示了DDIT3在Luminal A BC細胞增殖和TAM耐藥中起關鍵作用，并具有潛在的預后價值。綜上所述，dddit3可能通過調節TP63和PDGFR的表達而在luminal A型BC中發揮作用。

該論文中，HEK293T和人乳腺癌(BC)細胞系MCF-7的體外培養是使用Ausbian特級胎牛血清完成的。